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HelpChromosome Analysis, Products of Conception
Methodology
Includes microscopic examination of both chromosome number and structure. Analysis of 20 metaphases or 15 cones is routinely performed, if available. Additional metaphases are studied when indicated. Two or more G-banded karyotypes are created by computer image analysis. Special staining techniques are employed if needed. Routine light microscopy is also performed.Useful for the cytogenetic analysis of solid tissue from spontaneously miscarried pregnancies or from organ tissue taken at time of autopsy.
Performing Laboratory
Magee-Women's HospitalSpecimen Requirements
Chromosome Analysis1. Obtain 1 cm3 to 2 cm3 specimen of fetal tissue or villus material using aseptic technique and sterile instruments.
2. Place in sterile container, physiological saline, or balanced salt solution. The volume of solution only needs to cover the specimen to keep it moist.
3. Send specimen refrigerated. Specimen cannot be frozen. Forward promptly.
Note: 1. Label container with patient’s name (first and last), medical record number or date of birth, date and time of collection, specimen source, and solution the specimen was placed in (saline or balanced salt).
2. Contact Surgical Pathology Laboratory (412-344-6600, ext. 1441) to alert them that a specimen for chromosome analysis will be arriving.
3. Please complete highlighted areas on “Cytogenetic Testing Requisition” and complete a “Surgical Pathology/Cytology Request Form,” including the patient’s clinical history, diagnosis, and specimen source and forward with the specimen.
Routine Light Microscopy
1. Place remaining products of conception/fetal tissue in 10% neutral buffered formalin (NBF).
2. Forward promptly at ambient temperature.
Note: 1. Label container with patient’s name (first and last), medical record number or date of birth, date and time of collection, specimen source, and solution the specimen was placed in (10% NBF).
2. Contact Surgical Pathology Laboratory (412-344-6600, ext. 1441) to alert them that a specimen for routine tissue processing will be arriving.
3. Please complete highlighted areas on “Cytogenetic Testing Requisition” and a “Surgical Pathology/Cytology Request Form,” including the patient’s clinical history, diagnosis, and specimen source and forward with the specimen.
Reference Values
46,XX or 46,XYNo apparent chromosome abnormality.
An interpretive report will also be provided.