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Test Code BTR Isoagglutinin Titer, Anti-B, Serum


Shipping Instructions


Specimen must arrive within 10 days of collection.



Specimen Required


Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 2.5 mL

Pediatric Volume: 2 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Secondary ID

113384

Useful For

Evaluation of individuals with possible hypogammaglobulinemia

Method Name

Hemagglutination

Reporting Name

Isoagglutinin Titer, Anti-B

Specimen Type

Serum Red

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Ambient (preferred) 4 days
  Frozen  10 days
  Refrigerated  10 days

Reject Due To

Gross hemolysis OK

Clinical Information

Isoagglutinins are antibodies produced by an individual that cause agglutination of red blood cells (RBC) in other individuals. People possess isoagglutinins directed toward the A or B antigen absent from their own RBC. For example, type A or O individuals will usually possess anti-B. The anti-B is formed in response to exposure to B-like antigenic structures found in ubiquitous non-red blood cell biologic entities (eg, bacteria).

 

Isoagglutinins present in the newborn are passively acquired from maternal circulation. Such passively acquired isoagglutinins will gradually disappear, and the infant will begin to produce isoagglutinins at 3 to 6 months of age.

 

Isoagglutinin production may vary in patients with certain pathologic conditions. Decreased levels of isoagglutinins may be seen in patients with acquired and congenital hypogammaglobulinemia and agammaglobulinemia.

Reference Values

Interpretation depends on clinical setting. No defined reference values.

Interpretation

The result is reported as antiglobulin phase, in general representing IgG antibody. The result is the reciprocal of the highest dilution up to 1:1024 at which macroscopic agglutination (1+) is observed. Dilutions above 1:1024 are reported as greater than 1024.

Method Description

Two-fold serial dilutions of patient's serum are tested with appropriate type A and B erythrocytes. Antiglobulin phase of reactivity is examined. The result is the reciprocal of the highest dilution at which macroscopic agglutination (1+) is observed up to greater than 1024. Parallel titration of control antiserum is used for standardization.(Fung MK, Eder AF, Spitalnik SL, Westhoff CM: Technical Manual. 19th ed. AABB; 2017)

Day(s) Performed

Monday through Friday, Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86886

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BTR Isoagglutinin Titer, Anti-B 34464-8

 

Result ID Test Result Name Result LOINC Value
BTR Isoagglutinin Titer, Anti-B 34464-8

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.