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HelpTest Code C2729; SQ: CA27 Breast Carcinoma-Associated Antigen, Serum
Secondary ID
606583Useful For
Aiding in the management of breast cancer in patients with metastatic disease by monitoring the progression or regression of disease in response to treatment
Serial testing in women with prior stage II or III breast cancer who are clinically free of disease
May be useful for predicting early recurrence of disease in women with treated carcinoma of the breast
This test is not useful for screening women for or diagnosis of carcinoma of the breast.
Disease States
- Breast cancer
Method Name
Chemiluminometric Immunoassay
Reporting Name
Breast Carcinoma Assoc Ag(CA 27.29)Specimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial. Specimens that have not been aliquoted will be canceled.
2. Send refrigerated.
Specimen Minimum Volume
0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 7 days | |
| Frozen | 90 days | ||
| Ambient | 4 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
Clinical Information
Carcinoma of the breast is the most prevalent form of cancer in women. These tumors often produce mucinous antigens, which are large-molecular-weight glycoproteins with O-linked oligosaccharide chains.
Monoclonal antibodies directed against these antigens have been developed, and several immunoassays are available to quantitate the levels of tumor-associated mucinous antigens in serum. The antibodies recognize epitopes of a breast cancer-associated antigen encoded by the human mucin 1 (MUC-1) gene, which is known by several names including MAM6, milk mucin antigen, cancer antigen (CA) 27.29, and CA 15-3.
While CA 27.29 is expressed at the apical surface of normal epithelial cells, it is present throughout malignant epithelial cells of the breast, lung, ovary, pancreas, and other sites. The cancer-associated form of the antigen is less extensively glycosylated than the normal form and more specific for tumor cells.
Reference Values
Males
≥18 years: ≤38.0 U/mL (use not defined)
Females
≥18 years: ≤38.0 U/mL
Reference values have not been established for patients who are younger than 18 years of age.
Serum markers are not specific for malignancy, and values may vary by method.
Interpretation
Increased levels of cancer-associated antigen (CA 27.29) (>38 U/mL) may indicate recurrent disease in a woman with treated breast carcinoma and may be useful as an indication that additional tests or procedures should be performed to confirm recurrence.
Method Description
Cancer antigen (CA) 27.29 is measured using an automated, competitive, chemiluminescent immunoassay. The signal (Lite) reagent is a monoclonal antibody specific for CA 27.29, which is labeled with acridinium ester. Purified CA 27.29 antigen attached to paramagnetic particles (solid phase) competes with the antigen in the specimen for binding to the monoclonal antibody. An inverse relationship exists between the amount of CA 27.29 in the patient specimen and the amount of relative light units detected by the system.(Package insert: Advia Centaur XPT CA 27.29. Siemens Healthcare Diagnostics Inc.; 11206409_EN Rev. 22, 10/2022)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86300
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| C2729 | Breast Carcinoma Assoc Ag(CA 27.29) | 17842-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| C2729 | Breast Carcinoma Assoc Ag(CA 27.29) | 17842-6 |
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)
-Oncology Test Request (T729)
Highlights
The US Food and Drug Administration has approved CA 27.29 for serial testing in women with prior stage II or III breast cancer who are clinically free of disease.