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Test Code CMVGS; SQ: CMVIGG Cytomegalovirus Antibody, IgG, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial


Secondary ID

621835

Useful For

Determining whether a patient has had previous exposure to or recent infection with cytomegalovirus, including pregnant women.

 

This test is not useful for screening blood or plasma donors, or neonatal screening.

Highlights

Detection of IgG-class antibodies to cytomegalovirus (CMV) may be useful to assess the serological status of CMV.

Method Name

Electrochemiluminescence Immunoassay (ECLIA)

Reporting Name

CMV Ab, IgG, S

Specimen Type

Serum

Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 28 days
  Frozen  180 days
  Ambient  7 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Additives (eg, biocides, antioxidants) Reject

Clinical Information

Cytomegalovirus (CMV) is a member of the Herpesviridae family of viruses and usually causes asymptomatic infection after which it remains latent in patients, primarily within bone marrow-derived cells. Primary CMV infection in immunocompetent individuals may also manifest as a mononucleosis-type syndrome, similar to primary Epstein-Barr virus infection, with fever, malaise, and lymphadenopathy.

 

Cytomegalovirus is a significant cause of morbidity and mortality among bone marrow or solid organ transplant recipients, individuals with AIDS, and other immunosuppressed patients due to virus reactivation or a newly acquired infection. Infection in these patient populations can affect almost any organ and lead to multiorgan failure. CMV is also responsible for congenital disease among newborns and is one of the TORCH infections (toxoplasmosis, other infections including syphilis, rubella, CMV, and herpes simplex virus).

 

Cytomegalovirus seroprevalence increases with age. In the US, the prevalence of CMV specific antibodies increases from approximately 36% in children aged 6 to 11 years to over 91% in adults older than 80 years.(4)

Reference Values

Negative

Reference values apply to all ages.

Interpretation

Negative

Negative for cytomegalovirus (CMV) IgG. False negative results may occur in immunocompromised patients.

 

Borderline

Recommend follow-up testing in 10 to 14 days if clinically indicated.

 

Positive

Cytomegalovirus IgG antibodies detected, which indicate recent or remote infection.

Method Description

The electrochemiluminescence immunoassay for the in vitro quantitative determination of IgG antibodies to cytomegalovirus (CMV) in human serum is a sandwich test principle. During the first incubation, 12 mcL of sample, biotinylated recombinant CMV-specific antigens, and CMV-specific recombinant antigens labeled with a ruthenium complex form a sandwich complex. In the second incubation, streptavidin-coated microparticles are added and the complex becomes bound to the solid phase via interaction of biotin and streptavidin. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell II M. Application of a voltage to the electrode then induces chemiluminescent emission which is measured by a photomultiplier. Results are determined by a calibration curve which is instrument-specifically generated by 2-point calibration and a master curve provided via the cobas link.(Package insert: Elecsys CMV IgG, Roche Diagnostics GmbH, 01/2023)

Day(s) Performed

Monday through Saturday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86644

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CMVGS CMV Ab, IgG, S 22244-8

 

Result ID Test Result Name Result LOINC Value
GCMV CMV Ab, IgG, S 22244-8