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Test Code FDERM Fungal Culture, Dermal

Reporting Name

Fungal Culture, Dermal

Useful For

Recovery and identification of dermatophyte fungi from hair, skin, and nail infected specimens

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Shipping Instructions


Specimen must arrive within 7 days of collection.

 

Transport in petri dishes may result in loss of specimen. Securely tape petri dishes closed for transport.



Necessary Information


Specimen source is required.



Specimen Required


Note:

-Aseptic techniques should be used when collecting specimens to minimize contamination.

-For optimal recovery of organisms, sufficient clinical material should be collected.

 

Specimen Type: Hair

Container/Tube: Dry sterile container or specimen collection envelope

Specimen Volume: 10 to 12

Collection Instructions: Using forceps collect affected hairs with base of the shaft intact.

 

Specimen Type: Nails

Container/Tube: Dry sterile container or specimen collection envelope

Specimen Volume: Entire collection

Collection Instructions:

1. Wipe the nail with 70% alcohol using gauze (not cotton).

2. Clip away a generous portion of the affected area.

3. Collect material or debris from under the nail.

 

Specimen Type: Skin

Container/Tube: Dry sterile container or specimen collection envelope

Specimen Volume: Entire specimen

Collection Instructions:

1. Cleanse the affected area with 70% alcohol.

2. Gently scrape the surface of the skin at the active margin of the lesion, being careful to not draw blood.


Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient 7 days

Reference Values

Negative

If positive, fungus or yeast will be identified.

Day(s) Performed

Monday through Sunday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87101-Fungal culture, dermal

87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)

87107-Id MALDI-TOF Mass Spec Fungi (if appropriate)

87107-Fungal identification panel A (if appropriate)

87107-Fungal identification panel B (if appropriate)

87150-Identification rapid PCR Coccidioides (if appropriate)

87150 x 2-Identification Histoplasma/Blastomyces, PCR (if appropriate)

87153-D2 fungal sequencing identification (if appropriate)

87150-Id, Candida auris Rapid PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FDERM Fungal Culture, Dermal 580-1

 

Result ID Test Result Name Result LOINC Value
FDERM Fungal Culture, Dermal In Process

Clinical Information

Fungal infections of keratinized tissues (hair, skin, nails) can be caused by dermatophytic fungi belonging to the genera Epidermophyton, Microsporum, and Trichophyton. Opportunistic superficial infections resembling dermatophytoses may be caused by yeasts or by unrelated filamentous fungi that are normally saprobes or plant pathogens. Dermatophytes are usually unable to penetrate deeper tissues. Infection may range from mild to severe.

Interpretation

Positive cultures are reported with organism identification.

 

Negative reports are issued after 30 days incubation.

Method Description

Specimens are plated on mycobiotic agar, which contains chloramphenicol and cyclohexamide to inhibit bacterial and saprobic fungal contamination. Cultures are incubated at 30° C for 30 days. Identification of dermatophyte species is based on colony and microscopic morphology and polymerase chain-reaction (PCR), DNA sequencing or matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, when applicable.(Hall L, Wohlfiel S, Roberts GD: Experience with the MicroSeq D2 large-subunit ribosomal DNA sequencing kit for identification of filamentous fungi encountered in the clinical laboratory. J Clin Microbiol. 2004 Feb;42[2]:622-626; Theel ES, Hall L, Mandrekar J, Wengenack NL: Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2011 Dec;49[12]:4067-4071)

Reject Due To

Charcoal, wooden shaft, or dry swab
Agar plate
Blades from scalpel or razor		 Reject

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
FUNA Fungal Ident Panel A No, (Bill Only) No
FUNB Fungal Ident Panel B No, (Bill Only) No
LCCI Ident Rapid PCR Coccidioides No, (Bill Only) No
LCHB Id, Histoplasma/Blastomyces PCR No, (Bill Only) No
RMALF Id MALDI-TOF Mass Spec Fungi No, (Bill Only) No
RMALY Id MALDI-TOF Mass Spec Yeast No, (Bill Only) No
D2F D2 Fungal Sequencing Identification No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex test may be performed at an additional charge.

Method Name

Plated to Mycobiotic Agar

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.