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HelpTest Code FUNCUL Culture, Fungus, Lower Respiratory
Methodology
Conventional Culture Plated to SABHI, SABHI CC, and Brain/Heart Infusion with 10% Sheep Blood and Gentamicin AgarIncludes fungus smear and culture.
Performing Laboratory
St. Clair Hospital LaboratorySpecimen Requirements
Submit only 1 of the following specimens:
Bronchial Washings and Suctional Sputum
1. Aseptically collect 5 mL to 10 mL of fluid in a screw-capped, sterile container.
2. Maintain sterility and forward promptly at ambient temperature. Send specimen refrigerated if specimen storage is >1 hour.
Note: Specimen source is required on request form for processing.
Sputum
Aerosol-Induced
1. Collect 5 mL to 10 mL from an early-morning specimen on at least 3 consecutive days (1 per day) in a screw-capped, sterile container as follows:
A. Induce cough by inhalation of warm, aerosolized, sterile sodium chloride solution and collect aerosol-induced specimen.
2. Maintain sterility and forward promptly at ambient temperature. Send specimen refrigerated if specimen storage is >1 hour.
Note: Specimen source is required on request form for processing.
Deep Cough
1. Collect 5 mL to 10 mL from an early-morning specimen on at least 3 consecutive days (1 per day) in a screw-capped, sterile container as follows:
A. Instruct patient to brush his/her teeth and/or rinse mouth well with water.
B. Have patient remove dentures.
C. Instruct patient to take a deep breath, hold it momentarily, and cough deeply and vigorously into container and collect deep-cough specimen.
2. Maintain sterility and forward promptly at ambient temperature. Send specimen refrigerated if specimen storage is >1 hour.
Note: 1. Nasal secretion, saliva, or 24-hour collection is not acceptable.
2. Specimen source is required on request form for processing.
Reference Values
NegativeIf positive, fungus will be identified.