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Test Code FUNHSN Culture, Fungus, Hair, Nail, or Skin

Methodology

Conventional Culture Plated to SABHI, SABHI CC, and Brain/Heart Infusion with 10% Sheep Blood and Gentamicin Agar
Fungus smear is not included.

Performing Laboratory

St. Clair Hospital Laboratory

Specimen Requirements

Submit only 1 of the following specimens:

 

Hair
1. Use a Wood’s lamp during collection, if possible (some infected hairs exhibit fluorescence).
2. Pluck hairs which are broken off or appear diseased using sterile forceps.
3. If diseased hair stubs are not apparent, submit edges of scalp lesion as follows:
A. Cleanse scalp lesion with 70% alcohol to reduce bacteria and saprophytic fungi.
B. Scrape from the outer periphery or border of the lesion or involved area using a sterile scalpel.
4. Place specimen in a screw-capped, sterile container or in a plain, red-top tube.
5. Maintain sterility and forward promptly at ambient temperature only.
Note:  Specimen source is required on request form for processing.

 

Nail
1. Scrape out friable material beneath edge of nail or scrape or clip off portions of abnormal-appearing nail.
2. Place specimen in a screw-capped, sterile container.
3. Maintain sterility and forward promptly at ambient temperature only.
Note:  Specimen source is required on request form for processing.

 

Skin
1. Cleanse scalp lesion with 70% alcohol to reduce bacteria and saprophytic fungi.
2. Scrape from outer periphery or border of lesion or involved area.
3. Place scrapings in a screw-capped, sterile container or in a plain, red-top tube.
4. Maintain sterility and forward promptly at ambient temperature only.
Note:  Specimen source is required on request form for processing.

Reference Values

Negative (no fungus isolated after 28 days)
If positive, fungus will be identified.

Day(s) Test Set Up

Monday through Sunday