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Test Code FVAG Fungal Culture, Vaginal

Reporting Name

Fungal Culture, Vaginal

Useful For

Monitoring therapy for vulvovaginitis

 

Managing chronic recurring disease

 

Determining the etiology of infectious vaginitis when other tests have been uninformative

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Shipping Instructions


Specimen should arrive within 24 hours of collection.



Necessary Information


Specimen source is required.



Specimen Required


Specimen Type: Swab

Source: Vaginal secretions

Container/Tube: Culture transport swab (noncharcoal)

Collection Instructions:

1. Before collecting specimen, wipe away any excessive amount of secretion and discharge.

2. Obtain secretions from the mucosal membrane of the vaginal vault with a sterile swab.

3. If smear and culture are requested or both a bacterial culture and fungal culture are requested, collect a second swab to maximize test sensitivity.


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Ambient  7 days

Reference Values

Negative

 

If positive, yeast will be identified.

Day(s) Performed

Monday through Sunday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87102-Fungal culture, vaginal

87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)

87107-Id MALDI-TOF Mass Spec Fungi (if appropriate)

87107-Fungal identification panel A (if appropriate)

87107-Fungal identification panel B (if appropriate)

87150-Identification rapid PCR Coccidioides (if appropriate)

87150 x 2-Identification Histoplasma/Blastomyces, PCR (if appropriate)

87153-D2 fungal sequencing identification (if appropriate)

87150-Id, Candida auris Rapid PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FVAG Fungal Culture, Vaginal 51723-5

 

Result ID Test Result Name Result LOINC Value
FVAG Fungal Culture, Vaginal 51723-5

Clinical Information

Candidal vulvovaginitis is believed to be the most frequent or second most frequent vaginal infection. Depending on the geographical area, its prevalence in women is estimated to be in the range of 5% to 20%. Besides Candida albicans, Candida glabrata, and Candida tropicalis are the most frequently isolated Candida species both from vulvo-vaginitis patients and from healthy carriers.

Interpretation

Meaningful diagnosis of vaginal candidiasis requires that 1) yeast are demonstrable in the affected area and 2) clinical symptoms and signs are consistent with the disease. Since in up to 20% of healthy women, yeast cells are part of the normal vaginal flora, the presence of Candida on culture may be meaningless or misleading unless other clinical factors are considered.

Method Description

Specimens are plated on inhibitory mold agar that contains chloramphenicol to inhibit bacterial contamination. Cultures are incubated at 30° C for 3 days. Identification of Candida species is accomplished by a variety of physiologic, biochemical, or molecular tests.(Berkow EL, McGowan KL: Specimen collection, transport, and processing: Mycology. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. Vol 1. ASM Press; 2019:2016-2024)

Reject Due To

Viral transport media (including but not limited to M4, M5, BD viral transport media, thioglycolate broth)
Petri dish
Reject
   

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
D2F D2 Fungal Sequencing Identification No, (Bill Only) No
FUNA Fungal Ident Panel A No, (Bill Only) No
FUNB Fungal Ident Panel B No, (Bill Only) No
LCCI Ident Rapid PCR Coccidioides No, (Bill Only) No
LCHB Id, Histoplasma/Blastomyces PCR No, (Bill Only) No
RMALF Id MALDI-TOF Mass Spec Fungi No, (Bill Only) No
RMALY Id MALDI-TOF Mass Spec Yeast No, (Bill Only) No
LCCA Id, Candida auris Rapid PCR No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed at an additional charge.

Method Name

Plated to Inhibitory Mold Agar

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.