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HelpTest Code HAPT; SQ: HAPTOG Haptoglobin, Serum
Reporting Name
Haptoglobin, SUseful For
Confirmation of intravascular hemolysis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume:1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 28 days | |
| Frozen | 28 days | ||
| Ambient | 14 days |
Reference Values
30-200 mg/dL
Day(s) Performed
Monday through Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
83010
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HAPT | Haptoglobin, S | 46127-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| HAPT | Haptoglobin, S | 46127-7 |
Clinical Information
Haptoglobin is an immunoglobulin-like plasma protein that binds hemoglobin. The haptoglobin-hemoglobin complex is removed from plasma by macrophages and the hemoglobin is catabolized. When the hemoglobin-binding capacity of haptoglobin is exceeded, hemoglobin passes through the renal glomeruli, resulting in hemoglobinuria.
Chronic intravascular hemolysis causes persistently low haptoglobin concentration. Regular strenuous exercise may cause sustained low haptoglobin, presumably from low-grade hemolysis. Low serum haptoglobin may also be due to severe liver disease.
Neonatal plasma or serum specimens usually do not contain measurable haptoglobin; adult levels are achieved by 6 months.
Increase in plasma haptoglobin concentration occurs as an acute-phase reaction. Levels may appear to be increased in conditions such as burns and nephrotic syndrome. An acute-phase response may be confirmed and monitored by assay of other acute-phase reactants such as alpha-1-antitrypsin and C-reactive protein.
Interpretation
Absence of plasma haptoglobin may indicate intravascular hemolysis. However, congenital anhaptoglobinemia is common, particularly in African Americans. For this reason, it may be difficult or impossible to interpret a single measurement of plasma haptoglobin. If the assay value is low, the test should be repeated after 1 to 2 weeks following an acute episode of hemolysis. If all the plasma haptoglobin is removed following an episode of intravascular hemolysis and if hemolysis ceases, the haptoglobin concentration should return to normal in a week.
Low levels of plasma haptoglobin may indicate intravascular hemolysis.
Method Description
In this Siemens Nephelometer II method, the light scattered onto the antigen-antibody complexes is measured. The intensity of the measured scattered light is proportional to the amount of antigen-antibody complexes in the sample under certain conditions. If the antibody volume is kept constant, the signal behaves proportionally to the antigen volume.
A reference curve is generated by a standard with a known antigen content on which the scattered light signals of the samples can be evaluated and calculated as an antigen concentration. Antigen-antibody complexes are formed when a sample containing antigen and the corresponding antiserum are put into a cuvette. A light beam is generated with a light emitting diode (LED), which is transmitted through the cuvette. The light is scattered onto the immuno-complexes that are present. Antigen and antibody are mixed in the initial measurement, but no complex is formed yet. An antigen-antibody complex is formed in the final measurement.
The result is calculated by subtracting value of the final measurement from the initial measurement. The distribution of intensity of the scattered light depends on the ratio of the particle size of the antigen-antibody complexes to the radiated wavelength.(Instruction manual: Siemens Nephelometer II Operations Instruction Manual; Siemens, Inc; Version 2.4, 07/2019; Addendum to the Instruction Manual 2.3, 08/2017)
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | Reject |
| Gross icterus | OK |
Method Name
Nephelometry