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HelpTest Code MMLYP Antimicrobial Susceptibility Panel, Yeast, Varies
Infectious
Reporting Name
Susceptibility Panel, YeastUseful For
Determining in vitro quantitative antifungal susceptibility (minimum inhibitory concentration) of non-fastidious yeast
As an aid in the management of certain circumstances, such as:
-Refractory oropharyngeal infections due to Candida species in patients who appear to be experiencing therapeutic failure with standard agents at standard doses
-Invasive infections due to Candida species when the utility of azole antifungal agents is uncertain (eg, when the infection is due to a non-Candida albicans organism)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
VariesShipping Instructions
1. See Infectious Specimen Shipping Guidelines
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.
Necessary Information
Specimen source and suspected organism identification are required. If identification of organism is not provided, FUNID / Culture Referred for Identification, Fungus, Varies will be performed at an additional charge.
Specimen Required
Specimen Type: Organism in pure culture
Supplies: Infectious Container, Large (T146)
Container/Tube: Sabouraud's dextrose agar slant
Specimen Volume: Infecting yeast isolate
Collection Instructions: Organism must be in pure culture, actively growing. Do not submit mixed cultures.
Specimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Special Instructions
Reference Values
Results reported in mcg/mL
Day(s) Performed
Monday through Saturday
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87186
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MMLYP | Susceptibility Panel, Yeast | 29577-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MMLYP | Susceptibility Panel, Yeast | 29577-4 |
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| SYP | Susceptibility Yeast Panel | No, (Bill Only) | Yes |
Testing Algorithm
When this test is ordered, the susceptibility yeast panel will be performed at an additional charge. All yeast submitted will have susceptibility testing performed and billed as appropriate.
Clinical Information
Candida species are the fourth leading cause of nosocomial infections and are frequent causes of community-acquired infections.
Antifungal susceptibility testing may aid in the management of patients with invasive infections due to Candida species or patients who appear to be experiencing therapeutic failure.
The Clinical Laboratory Standards Institute has approved the use of a broth microdilution method for determining the susceptibility of Candida species.
Interpretation
The Clinical and Laboratory Standards Institute method, breakpoints, and interpretive criteria are used.
Method Description
Yeast susceptibility is determined by colorimetric microdilution broth method using TREK Sensititre YeastOne Y011 Susceptibility Plate. Each plate is setup with appropriate dilutions of antifungal agents and a colorimetric indicator. After inoculation with a standardized suspension of organism in inoculum medium and incubation at 35° C for 24 to 48 hours; the minimum inhibitory concentrations for the test organism are determined by observing the lowest antifungal concentration showing inhibition of growth as evidenced by no color change of the test medium.(Clinical and Laboratory Standards Institute [CLSI]: Performance Standards for Antifungal Susceptibility Testing of Yeasts. 2nd ed. CLSI supplement M60. CLSI; 2020; CLSI: Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. 4th ed. CLSI standard M27. CLSI; 2017)
Reject Due To
| Agar plate | Reject |
Method Name
Minimum Inhibitory Concentration (MIC)
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Highlights
Antifungals included in the assay are amphotericin B, anidulafungin, caspofungin, fluconazole, itraconazole, isavuconazole, rezafungin, micafungin, posaconazole, and voriconazole.