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Test Code RESBAT Respiratory Pathogen Array, Nasopharyngeal

Methodology

Multiplex Polymerase Chain Reaction (PCR)

Useful for -

Rapid detection of respiratory tract infections caused by:

-Adenovirus

-Coronavirus 229E, HKU1, NL63 and OC43

-Human Metapneumovirus

-Influenza A, A subtype H1, A subtype H3, A subtype H1-2009

-Influenza B

-Parainfluenza Virus1, Virus 2, Virus 3, Virus 4

-Human Rhinovirus/Enterovirus

-Respiratory Syncytial Virus

-Bordetella Pertussis

-Chlamydophila pneumoniae

-Mycoplasma pneumoniae

Location Performed

St Clair Hospital Laboratory

Specimen Requirements

* Specimen must arrive within 4 hours if transported at room temperature (18-30° C)

*  Specimen may be held for up to 3 days if transported and stored at refrigerator temperature (2-8° C)

 

Specimen Type: Nasopharyngeal swab placed in Viral Transport Media (VTM)

Collection Instructions:

Nasopharyngeal swab

1. Obtain BD Universal Viral Transport Collection Kit, 3ml.

2. Aseptically remove cap from vial.

3. Using the provided flexible, mini-tipped, flocked swab, insert swab into one nostril.

4. Rotate swab over surface of posterior nasopharynx.

5. Withdraw swab from collection site; insert swab into VTM transport tube.

6. Repeating procedure for the second nostril will deliver optimum combined sample.

7. Break swab shaft by bending it against the vial wall evenly at the pre-scored line.

8. Replace cap on vial and close tightly.

9. Forward promptly at room temperature.

10. Send specimen refrigerated if transport storage is > 4 hrs.

Day(s) Set Up

Monday through Sunday

Reference Values:

Negative (for all targets)