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HelpTest Code ZMMLS Antimicrobial Susceptibility, Aerobic Bacteria, Varies
Infectious
Reporting Name
Susceptibility, Aerobic, MICUseful For
Determining the in vitro susceptibility of aerobic bacteria involved in human infections
Testing Algorithm
When this test is ordered, the reflex tests may be performed at an additional charge.
All aerobically growing bacteria submitted will automatically have susceptibility testing performed and billed as appropriate. Antimicrobial agents appropriate to the organism and specimen source will be tested according to Mayo Clinic's practice and the laboratory's standard operating procedures.
If appropriate, testing for mecA will be performed by polymerase chain reaction (PCR) under MECAB / Methicillin Resistance Gene, mecA Test (Bill Only). Indications for mecA testing include inadequate growth by phenotypic antimicrobial susceptibility testing, lack of current organism breakpoints for oxacillin or cefoxitin, and assessment of discrepancies between cefoxitin and oxacillin phenotypic testing results.
In the event that an isolate of Helicobacter pylori does not grow from a client sample or does not grow for susceptibility testing, reflex testing for HPCR1 / Helicobacter pylori with Clarithromycin Resistance Prediction, Molecular Detection, PCR (Bill Only) may be added.
The following tables provide a listing of the antimicrobials routinely tested as well as antimicrobials that may be tested upon request. These tables are organized by isolate groups and are not all inclusive. Call 800-533-1710 and ask to speak to the Bacteriology Antimicrobial Susceptibility Testing Laboratory if the organism or antimicrobial of interest are not listed in these tables.
-Aerobic Gram-Negative Bacilli Antimicrobials
-Additional Gram-Negative Bacteria Antimicrobials
-Staphylococcus, Enterococcus, Bacillus, and Related Genera Antimicrobials
-Additional Gram-Positive Bacteria Antimicrobials
For test utilization options, see Helicobacter pylori Diagnostic Algorithm.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
VariesOrdering Guidance
Mayo Clinic Laboratories will not perform susceptibility testing on select agents (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis). Consult with your state health department or the Centers for Disease Control and Prevention regarding antimicrobial susceptibility testing of such isolates. For more information see www.selectagents.gov/sat/list.htm.
Shipping Instructions
1. See Infectious Specimen Shipping Guidelines
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.
Necessary Information
Organism identification and specimen source are required.
Specimen Required
Supplies: Infectious Container, Large (T146)
Container/Tube: Agar slant or other appropriate media
Specimen Volume: Organism in pure culture
Collection Instructions:
1. Perform isolation of infecting bacteria.
2. Organism must be in pure culture, actively growing. Do not submit mixed cultures.
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Frozen | |||
| Refrigerated | |||
Special Instructions
Reference Values
Susceptibility results are reported as minimal inhibitory concentration (MIC) in mcg/mL. Breakpoints (also known as clinical breakpoints) are used to categorize an organism as susceptible, susceptible-dose dependent, intermediate, resistant, or nonsusceptible according to breakpoint setting organizations, either the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EUCAST), as applicable.
In some instances, an interpretive category cannot be provided based on available data; therefore, the following comment will be included on the report: There are no established interpretive guidelines for agents reported without interpretations.
For information regarding CLSI and EUCAST susceptibility interpretations, see Susceptibility Interpretative Category Definitions.
Day(s) Performed
Monday through Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87077-Ident by MALDI-TOF mass spec (if appropriate)
87185-Beta lactamase (if appropriate)
87186-Antimicrobial Susceptibility, Aerobic Bacteria, MIC-per organism for routine battery (if appropriate)
87181-Susceptibility per drug and per organism for drugs not in routine battery (if appropriate)
87150-H pylori + Clarithro Resistance PCR (if appropriate)
87150-mecA PCR (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ZMMLS | Susceptibility, Aerobic, MIC | 50545-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ZMMLS | Susceptibility, Aerobic, MIC | 21070-8 |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
| BLA | Beta Lactamase | No, (Bill Only) | No |
| SUS | Susceptibility | No, (Bill Only) | No |
| HPCR1 | H pylori + Clarithro Resistance PCR | No, (Bill Only) | No |
| MECAB | mecA PCR Test, Bill Only | No, (Bill Only) | No |
Clinical Information
Antimicrobial susceptibility testing (AST) determines the minimal inhibitory concentration (MIC) of antimicrobial agents. The MIC is a measurement of the activity of an antimicrobial agent against an organism. It is defined as the lowest concentration of an antimicrobial agent that inhibits growth of the microorganism. Clinical breakpoints are derived from a number of data including:
-The pharmacokinetics/pharmacodynamics of an antimicrobial agent
-The MIC distribution of a large number of isolates
-Clinical outcome data for a patient population treated with the antimicrobial of interest
Antimicrobial susceptibility testing should be performed on pure culture isolates of pathogenic bacteria (or those potentially pathogenic in special situations) grown from specimens that have been appropriately collected so as not to confuse clinically significant isolates with normal or contaminating microbiota. Susceptibility testing is indicated for any organism that contributes to an infectious process warranting antimicrobial chemotherapy if its susceptibility cannot be reliably predicted from the organism's identity.
The MIC obtained during AST is helpful in indicating the concentration of antimicrobial agent required at the site of infection necessary to inhibit the infecting organism. For each organism-antimicrobial agent combination, the Clinical and Laboratory Standards Institute and/or the European Committee on Antimicrobial Susceptibility Testing provides interpretive criteria for determining whether the MIC should be interpreted as susceptible, susceptible dose dependent, intermediate, nonsusceptible, resistant, or epidemiological cutoff value if applicable.
Interpretation
A "susceptible" category result and a low minimum inhibitory concentration value indicate in vitro susceptibility of the organism to the antimicrobial tested.
Refer to Reference Values for interpretation of various antimicrobial susceptibility interpretive categories (ie, susceptible, susceptible-dose dependent, intermediate, nonsusceptible, resistant, or epidemiological cutoff value).
Method Description
An agar dilution method is used for routine testing. The agar dilution method employs the use of antimicrobial agents incorporated in agar plates. The antimicrobial is added to agar in various concentrations depending upon levels attainable in serum, urine, or both. A standardized suspension of the organism is applied to the agar plates, which are incubated for a minimum of 16 to 18 hours at 35° C. Complete inhibition of all but one colony or a very fine residual haze represents the end point.(Clinical and Laboratory Standards Institute [CLSI]: Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. 11th ed. CLSI standard M07.CLSI; 2018)
Daptomycin and tigecycline are tested by agar gradient diffusion.(Clinical and Laboratory Standards Institute [CLSI]. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. 11th ed. CLSI standard M07. CLSI; 2018; package insert: Etest Biomerieux;15203E-EN-2016/07, 07/2016)
Cefiderocol is tested by disk diffusion.(Clinical and Laboratory Standards Institute [CLSI]. Performance Standards for Antimicrobial Disk Susceptibility Tests. 13th ed. CLSI standard M02. CLSI; 2018)
Reject Due To
| Agar plate | Reject |
Method Name
Minimal Inhibitory Concentration (MIC) (Agar Dilution or Broth Microdilution or Gradient Diffusion) or Disk Diffusion(if appropriate)
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| MIC | Susceptibility, MIC | No, (Bill Only) | Yes |